Materials Needed:

1. Goat Grow Box: A mono-tub with grain spawn and substrate. (included in kit)

2. Cleaning supplies: 70% isopropyl alcohol, spray bottle, gloves, paper towels.

3. Spores or Mushroom Culture: Acquire a spore syringe or a mushroom culture.

4. Misting Bottle: For maintaining humidity.

5. Micropore Tape: To create a filtered air exchange. (included in kit)

6. Tub Liner: Prevents side-pinning and makes for easier cleanup. (included in kit)

7. Spoon: Clean spoon for mixing.

Procedure:

Step 1: Inoculate with Spores or Culture (Skip if Grain Spawn is colonized)

1. Prepare Inoculation Area:

1. • • • Create a clean workspace by wiping down surfaces you'll be working on with a disinfectant.

2. Inoculate Bag:

   • • • Inoculate each spawn bag with spores or a mushroom culture using a heat-sterilized needle or syringe. (Flame or induction heat to 185F)

3. Incubate Bag:

   • • • Place inoculated bag in a warm (75-78F), dark environment for the mycelium to colonize the substrate.

4. Break and Shake Bag:

• Once the mycelium has sufficiently colonized the grains but before it forms a solid mass, gently break apart the mycelial clumps by kneading, squeezing, or massaging the bag with clean hands.

• • Be careful not to damage the bag or create any holes that may introduce contaminants.

Step 2: Prepare the Mono-Tub , Grain Spawn and Substrate

1. Prepare Tub and Liner:

   • Clean the plastic tub, lid, and liner with 70% isopropyl alcohol to disinfect.

   • Place liner in bottom of tub.

2. Prepare Grain Spawn

1. • Clean the grain spawn bag with 70% isopropyl alcohol to disinfect.

1. • Once the mycelium has completely colonized the grain , gently break apart the mycelial clumps by kneading, squeezing, or massaging the bag with clean hands.

2. Prepare Substrate:

1. • Clean the mixing spoon with 70% isopropyl alcohol to disinfect.

1. • Using a disinfected spoon mix the colonized grain spawn with substrate bag

   • Add casing layer and spread to cover the grain and substrate mixture using only the tip of the spoon. Do Not mix casing layer into rest of mixture.

Step 3: Colonize Substrate

1. Place inoculated tub in a warm (75-78F), dark environment for the mycelium to colonize the substrate.

2. Monitor growth from outside tub.

3. Allow the tub to incubate in the dark until the mycelium has fully colonized the substrate.

Step 4: Initiate Fruiting Conditions

1. Introduce Light and Fresh Air:

   • Place the tub in a well-lit area with indirect light.

   • Fan the tub once a day to simulate fresh air exchange (about 15-30 seconds).

2. Maintain Humidity:

   • Mist the inside of the tub regularly to maintain high humidity levels.

Step 5: Harvest and Maintain

1. Harvest Mushrooms:

   • Harvest mushrooms when they reach maturity.

2. Continue Fruiting:

   • Continue to mist and maintain fruiting conditions until the harvest is complete.

Step 6: Drying Fruit

1. Place harvested fruit in dry, dark space until dry with plenty of air flow (the texture of a cracker)

2. Once dry, use your fingers or a small brush to clean substrate from fruit (optional)

Step 7: Clean and Repeat

1. Clean Equipment:

   • Clean and disinfect all equipment for future use.

2. Repeat Process:

   • Repeat the process with new substrate and spores or culture for continuous mushroom cultivation.

Notes:

• Follow sterile techniques throughout the process to minimize the risk of contamination.

• Consult additional resources and guides for more detailed information on mushroom cultivation techniques.